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Human Lipoarabinomannan, LAM ELISA Kit(96T)

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Quick Details

Brand Name: Sunlong
Product Spec: 96T
Instrument classification: Class II
Type: Blood Testing Equipments
Place of Origin: Zhejiang China
Purpose: For research
storage: 2-8℃
Model Number: SL1075Hu
Packaging Detail: Ice bags in foam box, carton box outside.
Selling Units: Single item
Single package size: 27X19X16 cm
Single gross weight: 2.0 KG
Package Preview: https://sc04.alicdn.com/kf/H9fbd8ceb0a234551a0764f42126e2b0c1.jpg_640x640.jpg,https://sc04.alicdn.com/kf/Ha75ff4b9fa544b06b5a253b4abb0f5d8l.jpg_640x640.jpg
Hangzhou Sunlong Biotech Co., Ltd.
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Product Description
  ELISA Kit  components

 

 Components48 T/box96 T/boxStorage
1User manual 1 copy1 copyR.T.
2Plate Cover 2pcs2pcsR.T.
3Sealed bags11R.T.
4Microplate1X481x492-8°C
5Standard0.5ml×1 bottle0.5ml×1 bottle2-8°C
6Standard diluent1.5ml×1 bottle1.5ml×1 bottle2-8°C
7Enzyme Conjugate3ml×1 bottle6ml×1 bottle2-8°C
8Sample diluent3ml×1 bottle6ml×1 bottle2-8°C
9Substrate Solution A3ml×1 bottle6ml×1 bottle2-8°C
10Substrate Solution  B3ml×1 bottle6ml×1 bottle2-8°C
11Stop Solution3ml×1 bottle6ml×1 bottle2-8°C
12Washing Buffer(20/30×)20ml (20X)×1bottle20ml (30X)×1bottle2-8°C

 

elisa kit

 

[Testing Process] 

 This kit employs Double Antibody Sandwich Technique. The principle of Double Antibody Sandwich is based on characteristics of the tested antigen with more than two valances which can identify coated antibody and detection antibody at same time. The specific process is as follows:

1. Connect the specific antibodies and solid phase carriers to form immobilized antibodies. Wash out uncombined antibodies and impurities. Seal the rest binding sites with irrelevant proteins.

2. Join under test with immobilized antibodies for contact reaction. After a while, combine antigens in and antibodies on carriers into the antigens complex. Wash out uncombined antibodies and impurities.

3. Add biotin labeling antibodies to combine with the antigens on immune complexes. Wash out the uncombined biotin labeling antibodies thoroughly. The enzyme amount on the carrier is now positively related to the amount of the tested substance in specimens.

4. Add horseradish peroxidase to label the avidins and incorporate them with the biotin labeling antibodies. Wash out the incorporated enzyme markers thoroughly. The enzyme amount on the carrier is now positively related to the amount of the tested substance in specimens.

5. Add substrates for coloring, and compute the concentration of specimens.

 

[Detection principle]

This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

 

[Schematic diagram  ]

 

 

[Intera assay Precision]≤ 8%  [Inter assay Precision]≤ 12% 

[Storage]-20°C [Short-term should be placed 4°C(such as two weeks)]

[Validity]12 months (-20°C).

 

 [Reference]

 

1 .Seigel, L.J.et al.(1984) Science 223:175.

 2. Minami, Y.et al.(1993) Annu. Rev. Immunol. 11:245.

 

Packaging & Shipping

Elisa kit packing method: all kits sent along with foam box,48T/BOX or 96T/BOX

Shipping:By TNT/DHL/UPS/FEDEX(Door to Door) and it take 3-5 days to reach your hand.

 

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Company Information

 

Our Team

 

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Our elisa kit animal breeding base

 

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Our elisa kit packing room 

 

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Our elisa kit technicist

 

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Our Exhabition

 

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Advantages

 

1. Thoustand kinds of  products can be provided

2. High-quality R&D team for product development and experienced technical team for aftersales sevice.

3.Products widely used in research and diagnostic area.Hot sale in Middle East, Europe, Africa.etc

4.Short delivery time 1-3 days after payment by DHLTNT UPS FEDEX 

Contact us

Contact us: crystal(@)sunlongbiotech.com

Any one who want to buy our elisa kits or want to be a distributor of us.Kindly email us to crystal(@)sunlongbiotech.com
Or say " Hi " to Whatsapp:+86 13738079864
Mob:+86  13738079864