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Multifunctional Assay Testing Malb Immunofluorescence Kit

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Port: China
Payment Terms: L/C,T/T,Western Union,MoneyGram
Supply Ability: 10000 Piece/Pieces per Month
Linear range: 5-300 mg/L
Place of Origin: Jiangsu China
Warranty: 3 years
Sample Volume: 10 μL
Specification: 25 pieces per box
Model Number: mAlb
After-sale Service: Online technical support
Brand Name: Norman
Instrument classification: Class II
Type: Immunoassay System
Accuracy: >90%
Sensitivity: 5 mg/L
Storage: Room temperature
Sample Type: Urine
Incubation time: 5 minutes
Application: Early detection of kidney disease
Certificate: CE/ISO13485/FSC
Packaging Detail: Paper box
Package Preview: https://sc04.alicdn.com/kf/H9f1991e008d14896a30458d61572e6ced.jpg_640x640.jpg
Nanjing Norman Biological Technology Co., Ltd.
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Product Description

 

[Intended use]

 Microalbumin (mAlb) Kit (Immunofluorescence) is mainly for the in vitro quantitative determination of Micro Albumin (mAlb) content in human. Microalbumin refers to the presence of trace albumin in the urine. Albumin is a normal protein in the blood, but only a very small amount of albumin appears in the urine under physiological conditions. Trace albumin urine reflects abnormal leakage of protein in the kidneys. The increase of urinary microalbumin is more common in diabetic nephropathy, hypertension and pre-eclampsia, and is an early sensitive index of renal injury. No matter which disease causes urinary microalbumin is due to different causes of the kidney inherent cell damage, so that the structure of the kidney inherent cells change, the function changes with the structure, the embodiment in the urine. The common detection methods in clinic are immunoturbidmetry method, immunofluorescence method, Colloidal gold method and Chemiluminescence method.

 

[Test principle]

 The assay is a double-antibody sandwich immunoassay for quantitative determination of mAlb concentration based on immunofluorescence technology. The sample droplets to be measured were added to the sample well of the detection card, Through chromatography, the sample reacts with a fluorescent latex coated with urinary microalbumin monoclonal antibody I on the conjugated pad. The reaction complex spread forward along the nitrocellulose membrane captured by the urinary microalbumin monoclonal antibody II, which was fixed on the test line. The more urinary microalbumin in the sample, the more complexes were accumulated on the test line, he intensity of the fluorescent antibody signal reflected the amount of urinary microalbumin captured. The concentration of urinary microalbumin in the sample can be determined by the Immunofluorescence quantitative analysis instrument produced by the company.

 

[Package]

 25 Tests /Box

 

[Materials provided]

 Contents

25        Cartridges

1         Calibration Card

25        Sample diluents

25        Disposable tips

 

[Storage and stability]

 Sealed: The kit must be stored at 10-30°C, valid for 18 months.

Opened: The cartridge must be used within 1 hour once its foil pouch is opened.

 

[Procedure]

Remove the cartridge (after reaching room temperature), draw in 10μL of the sample to sample diluent. After gently mixing the sample, add 90μL into the well and incubate it for 5 minutes in the reagent strip incubator.

After incubation insert the cartridge into the immunofluorescence quantitative analyzer(NRM-FI-1000) using the cartridge arrows as a guide, and click start, then the instrument will scan the cartridge automatically.

 

[Expected values/Reference range]

 The reference range study was conducted based on mAlb content of 95% of the distribution range of statistical analysis in 200 apparently healthy people, the result was as follows:

Expected values: urine: ≤20mg/L

Each laboratory should investigate the transferability of the expected values to its own patient population and if necessary determine its own reference ranges

 

Sample type Urine
Certificate CE/ISO13485/FSC
Sample Volume 10 μL
Accuracy >90%
Linear range 5-300 mg/L

 

 

 

 

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