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Hot Selling A1c Hemoglobin Hba1c Immunofluorescence Kit

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Port: China
Payment Terms: L/C,T/T,Western Union,MoneyGram
Supply Ability: 10000 Piece/Pieces per Month
Linear range: 4%-14%
Place of Origin: Jiangsu China
Warranty: 3 years
Sample type: Whole blood
Sample Volume: 10 μL
Specification: 25 pieces per box
Model Number: HbA1c
After-sale Service: Online technical support
Brand Name: Norman
Instrument classification: Class II
Type: Immunoassay System
Accuracy: >90%
Sensitivity: 4%
Storage: Room temperature
Incubation time: 5 minutes
Application: Blood sugar level
Certificate: CE/ISO13485/FSC
Packaging Detail: Paper box
Package Preview: https://sc04.alicdn.com/kf/Hb3f29787c2284445969faa3b0553bdddo.jpg_640x640.jpg
Nanjing Norman Biological Technology Co., Ltd.
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Hot Selling A1c Hemoglobin Hba1c Immunofluorescence Kit

Product Description

 

[Intended use]

Hemoglobin A1c (HbA1c) Kit (Immunofluorescence) is mainly for the in vitro quantitative determination of Hemoglobin A1c (HbA1c) content in human blood. Hemoglobin A1c (HbA1c) is a combination of hemoglobin and blood sugar in human blood, and is positively related to blood sugar concentration. The combination is irreversible. HbA1c disappears with the death of red blood cells (the life span of red blood cell is around 120 days), and therefore, it can reflect the average blood sugar levels in the first 2-3 months of blood collection. The increase of  HbA1c can change the affinity of erythrocyte to oxygen, making tissues and cells anoxia, accelerating the formation of cardiovascular complications; can cause the thickening of glomerular basal membrane, inducing diabetic nephropathy (DN); can cause the increase of blood lipids and blood viscosity, and is an important factor in the occurrence of cardiovascular disease. It can also significantly reduce the compliance of red blood cells (that is, the ability to deform), making it difficult for red blood cells to penetrate the walls of blood vessels and bring oxygen into the tissue. Therefore, it is of great significance to monitor HbA1c for disease control in diabetic patients, the prediction of complications, and the screening of diabetic patients. The common detection methods in Clinic are Turbidity method, immunofluorescence method, enzymatic method and high performance liquid chromatography (HPLC) method.

 

[Test principle]

The assay is a double-antibody sandwich immunoassay for quantitative determination of HbA1c concentration based on immunofluorescence technology. The sample droplets to be measured were added to the sample well of the detection card. Through chromatography, the sample reacts with the fluorescent latex coated with hemoglobin monoclonal antibody I on the conjugated pad. The complex spread forward along the nitrocellulose membrane captured by the HbA1c monoclonal antibody and hemoglobin monoclonal antibody II, which was fixed on the test line. The more HbA1c in the sample, the more complexes accumulated on the test line. The intensity of the fluorescent antibody signal reflected the amount of HbA1c and hemoglobin captured. The concentration of HbA1c in the sample can be determined by the Immunofluorescence quantitative analysis instrument produced by Norman Biological.

 

[Package]

25 Tests /Box

[Sample Type]

Whole blood

[Materials provided]

Contents

25        Cartridges

1         Calibration Card

25        Sample diluents

25        Disposable tips

[Storage and stability]

Sealed: The kit must be stored at 10-30°C, valid for 18 months.

Opened: The cartridge must be used within 1 hour once its foil pouch is opened.

 

[Procedure]

Remove the cartridge (after reaching room temperature), draw in 10μL of the sample to sample diluent. After gently mixing the sample, add 90μL into the well and incubate it for 5 minutes in the reagent strip incubator.

After incubation insert the cartridge into the immunofluorescence quantitative analyzer(NRM-FI-1000) using the cartridge arrows as a guide, and click start, then the instrument will scan the cartridge automatically.

 

[Expected values/Reference range]

The reference range study was conducted based on HbA1c content of 95% of the distribution range of statistical analysis in 200 apparently healthy people, the result was as follows:

Expected values: 4.0%-6.0%.

Each laboratory should investigate the transferability of the expected values to its own patient population and if necessary determine its own reference ranges.

 

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Sample type Whole blood
Sensitivity 4%
Accuracy >90%
Linear range 4%-14%

 

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